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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Bmp7

Plasmid

#201909

Purpose

in vitro transcription of RNA encoding FLAG-tagged Bmp7a

Depositor

Mary Mullins

Article

Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

Insert

bmp7 (bmp7a Zebrafish)

Tags

FLAG tag

Promoter

SP6

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa LCD

Plasmid

#197014

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (WT)

Plasmid

#197001

Purpose

Mammalian inducible expression construct of myc-tagged SPOP

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

-

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1optim_1676-2193_U

Plasmid

#147729

Purpose

Bacterial Expression of CtNot1optim_1676-2193

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

CtNot1optim_1676-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF1_F

Plasmid

#146281

Purpose

Bacterial Expression of DrUPF1

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF1 (upf1 Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF3b_F

Plasmid

#146284

Purpose

Bacterial Expression of DrUPF3b

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF3b (upf3b Zebrafish)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA_D

Plasmid

#146140

Purpose

Mammalian Expression of tubcDNA

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC33_D

Plasmid

#146141

Purpose

Mammalian Expression of tubcDNA-PTC33

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC33

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA-PTC62_D

Plasmid

#146142

Purpose

Mammalian Expression of tubcDNA-PTC62

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA-PTC62

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC62-deltaIntron3_D

Plasmid

#146146

Purpose

Mammalian Expression of tubgenomic-PTC62-delIntron3

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC62-delIntron3

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-767del664-673_J

Plasmid

#146671

Purpose

Bacterial Expression of HsPatL1_517-767-del664-673

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-767-del664-673 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1_70-770del399-516_J

Plasmid

#146682

Purpose

Mammalian Expression of HsPatL1_70-770del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_70-770del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPatL1-del399-516_J

Plasmid

#146686

Purpose

Mammalian Expression of HsPatL1-del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-delta_E

Plasmid

#146181

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-PTC64-V5His6_E

Plasmid

#146183

Purpose

Insect Expression of DmAdh-PTC64

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh-PTC64 (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-V5His6_E

Plasmid

#146184

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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