We narrowed to 33,163 results for: puromycin

1. AICSDP-63: ACTN2-mEGFP

   Plasmid

   #124607

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJL296

   Plasmid

   #243700

   Purpose

   A piggyBac vector containing a synthetic reporter with mNeonGreen2(11)

   Depositor

   Jonathan Weissman

   Article

   Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

   Insert

   mNeonGreen2(11)
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1α-Cre-2A-Puro

   Plasmid

   #207743

   Purpose

   Expression of the Cre recombinase linked to a puro selectable marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-2A-Puro
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-MCS1-2A-MCS2

   Plasmid

   #71782

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Kras Wt

    Plasmid

    #75282

    Purpose

    Expresses human Kras wild-type

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None. Wild-Type

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 Puro shRNA Scramble

    Plasmid

    #162011

    Purpose

    Lentiviral negative control vector containing scramble shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    scramble shRNA
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1 EGFP-p62 HRD

    Plasmid

    #207550

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-P62
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1 EGFP-LC3B HRD

    Plasmid

    #207551

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-LC3B
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1 EGFP-GABARAPL1 HRD

    Plasmid

    #207552

    Purpose

    Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-GABARAPL1
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIX403 RNaseH1

    Plasmid

    #237695

    Purpose

    Inducible lentiviral expression of RNaseH1

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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