Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,852 results for: Kars;

Clear keywords
Showing: 10021 - 10050 of 11852 results

  1. SLC9A3R2

    Plasmid
    #38948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

    Enlarge

  4. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

    Enlarge

  5. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

    Enlarge

  6. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

    Enlarge

  7. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

    Enlarge

  14. Clim Act MCH (CAM)

    Plasmid
    #104443
    Purpose
    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    actin
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104443-plasmid-map-sequence-id-200869

    Enlarge

  15. Clim EF1 MCH (CEM)

    Plasmid
    #104444
    Purpose
    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    EF1
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104444-plasmid-map-sequence-id-200864

    Enlarge

  16. Clim Tub MCH (CTM)

    Plasmid
    #104445
    Purpose
    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.
    Depositor
    Elena Casacuberta, Inaki Ruiz-Trillo
    Insert
    mCherry
    Use
    Expression in corallochytrium (single-celled euka…
    Promoter
    tubulin promoter
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    104445-plasmid-map-sequence-id-200865

    Enlarge

  19. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

    Enlarge

  20. SLC9A3R2

    Plasmid
    #38926
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

    Enlarge

  23. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

    Enlarge

  26. HaloTag-LacI-KRAB

    Plasmid
    #232638
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-KRAB
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232638-plasmid-map-sequence-id-462856

    Enlarge

  27. FUW-hR3-EGFP

    Plasmid
    #119231
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3-EGFP (FLT4 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    P hUbC
    Available Since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    119231-plasmid-map-sequence-id-231731

    Enlarge

  28. pLentiN

    Plasmid
    #37444
    Purpose
    Control lentiviral plasmid containing FLAG-HA tags
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    FLAG-HA
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 3, 2012
    Availability
    Academic Institutions and Nonprofits only

  29. PERK 7: PERK.K618A.9E10.pBABE

    Plasmid
    #21820
    Depositor
    David Ron
    Insert
    PERK K618A (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    kinase dead K618A mutant
    Available Since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21820-plasmid-map-sequence-id-10016

    Enlarge

  30. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid
    #64035
    Purpose
    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
    Depositor
    Karel Svoboda
    Insert
    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    Use
    AAV
    Tags
    ReaChR tagged with mCitrine
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10021 - 10050 of 11852 results