We narrowed to 7,569 results for: GFP expression plasmids
-
Plasmid#154141PurposeExpresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianMutationR887EPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIC306
Plasmid#52257PurposeRetroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)DepositorInsertSka3 (SKA3 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9
Plasmid#201949PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en31FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationG1243T, E1369R, E1603H on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en1FnCas9
Plasmid#201947PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en1FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1369R on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-FnCas9ABEmax8.17d
Plasmid#201955PurposeMammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A on FnCas9, V82S, V106W, Q154R on mutant TadAPromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gThy1-CBh-mCherry-SV40late
Plasmid#249126Purposeexpression of gThy1 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1)
Plasmid#191341PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVPromoterEF1alpha and mU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late
Plasmid#249125Purposeexpression of gTgfbr2 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gTnfrfs1a-CBh-mCherry-SV40late
Plasmid#249127Purposeexpression of gTnfrsf1a and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-KIKO-U6-gOsmr-p2a-mCherry-STOP-pA-400
Plasmid#249130Purposeall-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gOsmr cut site, disrupting Osmr expressionDepositorInsertgOsmr, mCherry (Osmr Mouse, Synthetic)
UseAAVAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-U6-gCtnnb1-CBh-mCherry-SV40late
Plasmid#249119Purposeexpression of gCtnnb1 and mCherryDepositorAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
ρ1-EM GABAA receptor in pEZT-BM
Plasmid#213072PurposeExpress human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICDDepositorInsertHuman GABAa rho1 receptor (GABRR1 Human)
UseBacmam, baculovirus, oocyte expressionTagsTwin-Strep tag and superfolderGFPExpressionMammalianMutationEncodes residues S58–L383 and D451–S479, separate…PromoterCMV and T7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FHUG+W (B2)
Plasmid#74011Purposelentiviral expression of EGFP and shRNA (from H1 promoter, empty)DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
NaChBac pTracer CMV2
Plasmid#60835PurposeMammalian expression plasmid containing NaChBac gene with an EGFP reporterDepositorInsertNaChBac
ExpressionMammalianPromoterCMVAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald
Plasmid#214469PurposeLentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0248
Plasmid#162313PurposeReporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiTDepositorInsertT7prom-RBS-sfGFP-HiBiT
UseSynthetic BiologyAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only