We narrowed to 18,592 results for: MUT

1. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG-CMV-Tlr4(Lps)

   Plasmid

   #27149

   Depositor

   Bruce Beutler

   Article

   Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

   Insert

   toll-like receptor 4(Lps) (Tlr4 Mouse)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed Proline 712 to Histidine. Native signal p…

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pSOMA-T679A-NES

   Plasmid

   #118940

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-BAP1-N102S-GFP

   Plasmid

   #78915

   Purpose

   Mammalian expression of BAP1 with N102S and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

   Insert

   BAP1 (BAP1 Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   N102S

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMADM-alpha

   Plasmid

   #36890

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdTomato-3Myc
   beta-globin intron
   Neo
   ATG (T)
   GFP
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, deleted…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/HisMaxB-YAP1-S163A

   Plasmid

   #18989

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein S163A mutant (YAP1 Human)
   

   Tags

   His

   Expression

   Mammalian

   Mutation

   Serine 163 changed to Alanine,

   Available Since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCK669

   Plasmid

   #192638

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dxCas9-NG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dxCas9-NG h…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

   Plasmid

   #124299

   Purpose

   Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   UBE2G1 (UBE2G1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-VprBP (RARA)

   Plasmid

   #133587

   Purpose

   Expresses human VprBP RARA mutant in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   Vpr (HIV-1) binding protein (DCAF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   RARA mutation of human VprBP defective in DDB1 bi…

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/HisMaxB-YAP1-S164A

    Plasmid

    #18990

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein S164A mutant (YAP1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    Serine 164 changed to Alanine

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pMCSF-R(m40)-luc

    Plasmid

    #12425

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Mutation

    PU.1 binding site mutation (created a KpnI site i…

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pMinDis.X513-iNicSnFR3b-(V7)-PM

    Plasmid

    #125124

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)-PM
    

    Tags

    Ig-K-Chain and membrane anchoring protein-PDGFR

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CS-154

    Plasmid

    #166230

    Purpose

    lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

    Depositor

    Nozomu Yachie

    Article

    Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

    Inserts

    U6-gRNA EGFP targetting
    nCas9-PmCDA1-UGI-2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Promoter

    CMV promoter and Human U6

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Flag-EcoCas6

    Plasmid

    #106274

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIKAT111

    Plasmid

    #69996

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Expression

    Mammalian

    Mutation

    C1459T (R487C)

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRR.6

    Plasmid

    #69997

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6 variant

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Expression

    Mammalian

    Mutation

    Q172H, K262R

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRY-tTA (B702)

    Plasmid

    #92032

    Purpose

    Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    tTA2 (TetR fused to VP64)

    Expression

    Mammalian

    Mutation

    NLS on CRY2 is mutated

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-DmNot1_J

    Plasmid

    #146709

    Purpose

    Insect Expression of DmNot1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmNot1 (Not1 Fly)
    

    Expression

    Insect

    Mutation

    Three non silent mutations N605S, K759M, G1999S a…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDual-eGFP(W66)

    Plasmid

    #63217

    Purpose

    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(W66)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed tyrosine at position 66 with respect to t…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only