We narrowed to 150,751 results for: ins

1. MSP10-bio

   Plasmid

   #47719

   Purpose

   Expresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised MSP10
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV CAG ChR2 E123T T159C 2A tDimer

   Plasmid

   #85399

   Purpose

   High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   CAG and ribosomal skip sequence 2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+) 6xHis-TEV-IFIT2

   Plasmid

   #53558

   Purpose

   bacterial expression of IFIT2

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT2 (IFIT2 Human)
   

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

   Plasmid

   #35508

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

   Plasmid

   #182359

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Ultra-pan-neuronal positive control pUNP::gfp(NLS)
   

   Expression

   Worm

   Promoter

   Promoter Ultra Pan Neuronal

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. YW249_Pzipt17-loxP-H2BGFP-miniMOS

   Plasmid

   #196063

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   H2B::GFP
   NeoR
   Minimal Mos1
   zipt17p::zipt17::zipt17 3UTR
   

   Expression

   Worm

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX SHP-1(C)-SH2

   Plasmid

   #46494

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-1(C) (PTPN6 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 105-217

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-mCherry-C1-iCoChR++

   Plasmid

   #98173

   Purpose

   artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iCoChR++ gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX Crk-SH2

    Plasmid

    #46418

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Crk (CRK Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-124

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. SLK_HUMAN_D0

    Plasmid

    #79733

    Purpose

    This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SLK (SLK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pEMS1725

    Plasmid

    #105870

    Purpose

    High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2-FFrt-KanR-FFrt
    

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCST-AausFP2

    Plasmid

    #129500

    Purpose

    Expresses AausFP2 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP2
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-HA-TEV

    Plasmid

    #105769

    Purpose

    Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    HA-TEV

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX p85a(C)-SH2

    Plasmid

    #46463

    Depositor

    Leszek Kotula, Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(C) (PIK3R1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 612-724

    Promoter

    Tac

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pf113-bio

    Plasmid

    #47729

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf113
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG5-HA-ERR3 S57,81,219A

    Plasmid

    #37850

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serines 57, 81, and 219 to Alanine (AGC t…

    Promoter

    SV40

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid

    #184334

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXD4 (murine) MSCV

    Plasmid

    #8549

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Retroviral

    Tags

    His and T7

    Expression

    Mammalian

    Mutation

    contains T7 and HIS tags from pET; makes protein …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only