We narrowed to 14,682 results for: NTS;

1. CaV1.3e[8a,11,31b,Δ32,42a] mut

   Plasmid

   #26576

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pECE HA AMPKa1 AS

   Plasmid

   #69831

   Purpose

   Expresses Expresses AMPK alpha 1 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.E2.ChETA

   Plasmid

   #220310

   Purpose

   Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

   Depositor

   Matt Rowan

   Article

   Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

   Insert

   ChETA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   S5E2

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human b4 nicotinic receptor subunit EM construct

   Plasmid

   #163961

   Purpose

   pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human beta4 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   StrepII tag and apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Pro341-Ser395 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPKm-105

   Plasmid

   #104853

   Purpose

   pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   PhyB NT GBD
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

   Plasmid

   #74606

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A, W120A

   Promoter

   SFFV

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUW TetO HA-AMPKa1 AS

   Plasmid

   #69825

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4 3xFLAG Halo delta 122-130 nsp1

    Plasmid

    #175425

    Purpose

    Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 122-130 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-AuroraB L154G/H250Y

    Plasmid

    #108492

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1759

    Plasmid

    #154315

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::TEV::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Promoter

    U6

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid

    #74603

    Purpose

    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUW TetO NET1A-V5 WT

    Plasmid

    #69828

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge