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Plasmid#212901PurposeEncodes S.cerevisiae SUC2 (Invertase) pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD021
Plasmid#212907PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFapp8) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD024
Plasmid#212910PurposeEncodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD042
Plasmid#212912PurposeEncodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertHSP150 (HSP150 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSUC2 (SUC2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD044
Plasmid#212914PurposeEncodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD022
Plasmid#212908PurposeEncodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD009
Plasmid#212906PurposeEncodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSRL1 (SRL1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RS02060_pMQ30_KO_Kan
Plasmid#185391PurposeNon-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11DepositorInsertsRR42_RS02060 upstream flanking homology region
RR42_RS02060 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS28935_pMQ30_KO_Kan
Plasmid#185392PurposeNon-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11DepositorInsertsRR42_RS28935 upstream flanking homology region
RR42_RS28935 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS18965_pMQ30_KO_Kan
Plasmid#185394PurposeNon-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11DepositorInsertsRR42_RS18965 upstream flanking homology region
RR42_RS18965 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS18970_pMQ30_KO_Kan
Plasmid#185395PurposeNon-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11DepositorInsertsRR42_RS18970 upstream flanking homology region
RR42_RS18970 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF18_TurboID_3xHA
Plasmid#200017PurposeExpresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHADepositorInsertORF18
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF30_TurboID_3xHA
Plasmid#200018PurposeExpresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHADepositorInsertORF30
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF31_TurboID_3xHA
Plasmid#200019PurposeExpresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHADepositorInsertORF31
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF66_TurboID_3xHA
Plasmid#200020PurposeExpresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHADepositorInsertORF66
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_3xHA_TurboID_ORF34
Plasmid#200021PurposeExpresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboIDDepositorInsertORF34
UseTags3xHA-TurboIDExpressionMammalianMutationPromoterCMV promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo G226A ORF29
Plasmid#200024PurposeLentiviral vector for expression of KSHV ORF29 with G226A mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlycine 226 changed to alaninePromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:WNFLS2-2
Plasmid#202190PurposeExpress 'Washington navel' Orange FLS2-2 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:WNFLS2-1
Plasmid#202189PurposeExpress 'Washington navel' Orange FLS2-1 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R14A/K310A
Plasmid#162655PurposeExpresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids R14 and K310 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-2xStrep-ORF68 C52A
Plasmid#162660PurposeLentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cellsDepositorInsertORF68
UseLentiviralTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acid C52 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A
Plasmid#162639PurposeExpresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cellsDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag IIExpressionMammalianMutationAmino acids K174, R179, and K182 mutated to alani…PromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K435A
Plasmid#162651PurposeExpresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid K435 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 K450A/R451A
Plasmid#162653PurposeExpresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acids K450 and R451 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-optoJNKi-WPRE
Plasmid#159968Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activationDepositorInsertmScarlet-optoJNKi
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optoJNKi-WPRE
Plasmid#159966Purposeoptogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivityDepositorInsertmTurquoise2-optoJNKi
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-Ypet-optoJNKi-WPRE
Plasmid#159967Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activationDepositorInsertYpet-optoJNKi
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-181-2xStrep
Plasmid#138444PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-181PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-191-2xStrep
Plasmid#138445PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-191PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24 1-216-2xStrep
Plasmid#138446PurposeExpresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationAmino acids 1-216PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-mu24-2xStrep
Plasmid#138435PurposeExpresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertmu24
UseTagsStrepExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORF18(R158A_L159A)-3xFLAG pCDNA4
Plasmid#129814PurposeExpresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Arginine 158 to Alanine and Leucine 159 t…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Thy1.1-muSOX D219A
Plasmid#131704Purposeencodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cellsDepositorInsertThy1.1-muSOX
UseTagsExpressionMammalianMutationAspartic acid 219 mutated to an alaninePromoterCMVAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(G228A)-3xFLAG pCDNA4
Plasmid#129809PurposeExpresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Glycine 228 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(L29A)-3xFLAG pCDNA4
Plasmid#129777PurposeExpresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 29 to AlaninePromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only