We narrowed to 4,522 results for: ER

1. mEmerald-Sec23A

   Plasmid

   #166893

   Purpose

   mammalian expression of Sec23A tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Sec23A

   Plasmid

   #166894

   Purpose

   mammalian expression of Sec23A tagged with mCherry

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS003-lenti-CMV-GCaMPer

   Plasmid

   #65227

   Purpose

   ER calcium sensor for lentivirus production

   Depositor

   Adam Cohen, Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   GCaMPer
   

   Use

   Lentiviral

   Tags

   circularly permuted GFP

   Expression

   Mammalian

   Mutation

   GCaMP3 (D324G, D360G, D397G, D435G)

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Halo-Sec31

   Plasmid

   #166895

   Purpose

   mammalian expression of Sec31 tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   Sec31
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPLN-hFluc-WT-HA-GFP11-KDEL

   Plasmid

   #177722

   Purpose

   Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   GFP11, HA, KDEL, and Prolactin Signal Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sar1b-H79G-YFP

   Plasmid

   #166898

   Purpose

   mammalian expression of Sar1b-H79G tagged with YFP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   Sar1b-H79G
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   H79 to G

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xFKBP-mCh-KDEL

   Plasmid

   #173010

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-mCh-KDEL
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-Sec23A

   Plasmid

   #166892

   Purpose

   mammalian expression of Sec23A tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-mKate2-KDEL-pA

    Plasmid

    #109546

    Purpose

    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sar1b-H79G-mOrange2

    Plasmid

    #166900

    Purpose

    mammalian expression of Sar1b-H79G tagged with mOrange2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sar1b
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    H79G

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-X1-Neo-HA-UFL1-RAMP4

    Plasmid

    #139863

    Purpose

    Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFL1-RAMP4 (UFL1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    recombinant fusion of UFL1 (full length) with RAM…

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid

    #177723

    Purpose

    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFlucDM
    

    Use

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    R188Q, R261Q (Destabilized Mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFsynW KDEL Hook

    Plasmid

    #197277

    Purpose

    Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    Streptavidin
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    mCherry*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. ERroGFP_iE_C204Q_pCDNA3

    Plasmid

    #48629

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B, C204Q

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Use

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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