We narrowed to 737 results for: Tcta

1. pLCHKOv3-mClover-intergenic_4-As

   Plasmid

   #218816

   Purpose

   Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-U6gRNA5(MYC_2)-PGKpuroBFP-W

   Plasmid

   #211970

   Purpose

   Express gRNA against MYC with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting MYC (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MDM4_Deletion_Downstream_gRNA_1

   Plasmid

   #195136

   Purpose

   gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Downstream gRNA 1 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-sgBrip1#1/Cre

   Plasmid

   #193203

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgBrip1#1 (Brip1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-EGFP MASTL E167D (siRNA resistant)

   Plasmid

   #191013

   Purpose

   Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E167D

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-sgKmt2c#1/Cre

   Plasmid

   #173652

   Purpose

   Expresses a Kmt2c-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Kmt2c (Kmt2c Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-sgErcc4#1/Cre

   Plasmid

   #173593

   Purpose

   Expresses a Ercc4-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Ercc4 (Ercc4 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12

   Plasmid

   #129064

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA12 (mouseTSDR)
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgRNA targeting human SLC12A5 gene stop codon

   Plasmid

   #158577

   Purpose

   For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   sgRNA targeting human SLC12A5 gene stop codon
   

   Use

   CRISPR

   Tags

   Cas9 and GFP

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Blast-sgMARK3

    Plasmid

    #138706

    Purpose

    Expresses a human MARK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgMARK3 human (MARK3 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TLCV2-Exoc7-Ex5&10

    Plasmid

    #133303

    Purpose

    EGFP reporter sequence was removed from TLCV2 vector and two gRNAs targeting mouse Exoc7 gene were cloned into the modified TLCV2 vector.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Insert

    Exocyst complex component 7 (Exoc7 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX459 mDnd1#3

    Plasmid

    #106347

    Purpose

    small guide RNA #3 against RRM-coding region of Dnd1 gene locus

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C17orf75 gRNA (BRDN0001146368)

    Plasmid

    #78078

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human C17orf75

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    C17orf75 (C17orf75 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GLYCTK gRNA (BRDN0001146438)

    Plasmid

    #77907

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GLYCTK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GLYCTK (GLYCTK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BMPR1B gRNA (BRDN0001145690)

    Plasmid

    #77488

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BMPR1B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BMPR1B (BMPR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BMPR1B gRNA (BRDN0001149274)

    Plasmid

    #77489

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BMPR1B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BMPR1B (BMPR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DGKZ gRNA (BRDN0001147025)

    Plasmid

    #77466

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKZ

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKZ (DGKZ Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAP3K9 gRNA (BRDN0001145380)

    Plasmid

    #77308

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP3K9

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP3K9 (MAP3K9 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DCLK3 gRNA (BRDN0001144803)

    Plasmid

    #77311

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DCLK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DCLK3 (DCLK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IPPK gRNA (BRDN0001146016)

    Plasmid

    #77152

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IPPK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IPPK (IPPK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge