Addgene

We narrowed to 2,163 results for: Xpa

Showing: 661 - 680 of 2163 results

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pBS B2rescue A70D

Plasmid

#78658

Purpose

Generates B2 inserts suitable for rescue

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

Insert

B2rescue cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PB-Pitx2

Plasmid

#60668

Purpose

PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Pitx2 (Pitx2 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ3: LexA-VHH-Control

Plasmid

#108241

Purpose

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMC3-yeGFP

Plasmid

#176181

Purpose

yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

yEGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MCS and YFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBM13-sd4U

Plasmid

#179691

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)

Use

Tags

Expression

Bacterial

Mutation

Q1310R, S1330I, T1380I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST2-sfGFP

Plasmid

#126575

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFP.R271

Plasmid

#138220

Purpose

Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_PTS2

Plasmid

#65676

Purpose

Expresses PTS2-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_PCP-VP64_B-PP7

Plasmid

#177157

Purpose

Expresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

PCP-VP64
B-PP7

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_H2B

Plasmid

#65702

Purpose

Expresses YNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI3up

Plasmid

#176170

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI5up

Plasmid

#176172

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI5dw

Plasmid

#176173

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-5

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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