We narrowed to 253,018 results for: ins

1. FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

   Plasmid

   #58432

   Purpose

   Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)1-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRP800_insul-(lexA-box)3-PminCYC1-Citrine-TCYC1

   Plasmid

   #58436

   Purpose

   Induces Citrine expression from a promoter containing 3 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)3-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEA1-Nuclease-Puro HEK3 CTT ins

   Plasmid

   #171995

   Purpose

   Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLK89 - Edit-directing plasmid cloning insert

   Plasmid

   #98846

   Purpose

   Contains the insert used for step 2 of edit-directed plasmid cloning

   Depositor

   Leonid Kruglyak

   Insert

   KanR
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Changed sequence to remove a MluI cut site

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmSMG6-PINSMG5-siRNAres-V5His6_E

   Plasmid

   #146240

   Purpose

   Insect Expression of DmSMG6-PINSMG5-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

   Insert

   DmSMG6-PINSMG5-siRNAres (Smg6 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABEpuro-ERBB2 A775_G776insYVMA

   Plasmid

   #40982

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A775_G776insYVMA

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-EGFR-H773_V774insNPH

   Plasmid

   #116262

   Purpose

   Lentiviral expression of EGFR H773_V774insNPH

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H773_V774insNPH

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EGFR-A763_Y764insFQEA

    Plasmid

    #116233

    Purpose

    Lentiviral expression of EGFR A763_Y764insFQEA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A763_Y764insFQEA

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EGFR-M766_A767insAI

    Plasmid

    #116279

    Purpose

    Lentiviral expression of EGFR M766_A767insAI

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    M766_A767insAI

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXT128_TwinStrep-SUMO-ShCas12k

    Plasmid

    #135524

    Purpose

    Protein expression construct for ShCast12k

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShCas12k
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXT130_TwinStrep-SUMO-ShTnsC

    Plasmid

    #135526

    Purpose

    Protein expression construct for ShTnsC

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsC
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXT131_TwinStrep-SUMO-ShTniQ

    Plasmid

    #135527

    Purpose

    Protein expression construct for ShTniQ

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTniQ
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXT129_TwinStrep-SUMO-ShTnsB

    Plasmid

    #135525

    Purpose

    Protein expression construct for ShTnsB

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid

    #232344

    Purpose

    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF Like 2 (HDGFL2 Human)
    

    Use

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    TDP-43 regulated cryptic exon

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

    Plasmid

    #232343

    Purpose

    Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF like 2 (HDGFL2 Human)
    

    Use

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid

    #197264

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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