We narrowed to 7,519 results for: lenti crispr

1. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Direct-seq_lentiGuide-Puro-Loop2-8A8G

   Plasmid

   #157983

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Loop2-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Direct-seq_lentiGuide-Puro-Tail-8A8G

   Plasmid

   #157981

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCas9-T2A-mApple

   Plasmid

   #193780

   Purpose

   Tricistronic expression of Cas9-mApple-BSD in mammalian cells

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   mApple
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA KanR neo

   Plasmid

   #167909

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB700 lenti gRNA KanR neo_zhang2.0

   Plasmid

   #167917

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiGuideSLC35C1-neo

   Plasmid

   #220868

   Purpose

   encodes CRISPR sgRNA targeting human SLC3C1

   Depositor

   Jennifer Kohler

   Insert

   SLC35C1 (SLC35C1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYJ33-lenti-U6-Oct4-PE-acti-gRNA-DsRed

   Plasmid

   #131076

   Purpose

   Activation of Mafa via SAM system

   Depositor

   Xiaojun Lian

   Insert

   OCT4 proximal enhancer gRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCas9-BFP

    Plasmid

    #78545

    Purpose

    Fusion protein Cas9+BFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid

    #217330

    Purpose

    Lentiviral expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCH4 (denAsCas12a-KRAB lenti)

    Plasmid

    #217331

    Purpose

    Lentiviral expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuide_puro-P2A-mNeonGreen

    Plasmid

    #221464

    Purpose

    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker

    Depositor

    James Eshleman

    Insert

    mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6, EF-1a

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

    Plasmid

    #192676

    Purpose

    Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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