We narrowed to 10,563 results for: ESP

1. GFP-ataxin-3 N-t

   Plasmid

   #89980

   Purpose

   Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   construct contains aa 1-182; deleted aa 183-361

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCD1Dsol

   Plasmid

   #102955

   Purpose

   Mammalian expression of human soluble CD1D cDNA

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

   Insert

   Human soluble CD1D cDNA (CD1D Human)
   

   Tags

   BirA

   Expression

   Mammalian

   Mutation

   Lacks the transmembrane region

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-ataxin-3 C-t

   Plasmid

   #89981

   Purpose

   Expresses the GFP-tagged C-terminus of ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-182

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV TIP60 212-364

   Plasmid

   #78795

   Purpose

   To overexpress TIP60 212-364 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   TIP60 (KAT5 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   212-364, G214A

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-HA-BHLHE40(S243A/E248A)

   Plasmid

   #110155

   Purpose

   Expression of human BHLHE40 (S243A/E248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S243A/E248A

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 Flag MFF iso5 S172A

   Plasmid

   #74382

   Purpose

   Flag tagged human S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

7. p426_Cas9_gRNA-R-ARS607c

   Plasmid

   #87409

   Purpose

   p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 Flag MFF iso5 S155,172A

   Plasmid

   #74383

   Purpose

   Flag tagged human S155,172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   See 155 Ala, Ser 172 Ala (numbering based on MFF …

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRRLsin.PPT.CMV­‐GFP­‐Eps8 WT

   Plasmid

   #74922

   Purpose

   Lentiviral plasmid expressing Eps8 fused to GFP

   Depositor

   Giorgio Scita

   Article

   Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

   Insert

   Eps8 (Eps8 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. CmCitrine Lck w/ NosUTRs

    Plasmid

    #66785

    Purpose

    expression of fluorescent membranes in sea urchin primordial germ cells

    Depositor

    Amro Hamdoun

    Article

    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

    Insert

    Lck (LCK Human)
    

    Use

    Sea urchin, zebrafish, xenopus

    Tags

    3' UTR from sea urchin Nanos, 5' UTR fr…

    Mutation

    Tandem repeat of LCK the N-terminal membrane targ…

    Promoter

    T7

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid

    #89890

    Purpose

    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    HA-BirA-2A-citrine
    

    Use

    Unspecified

    Tags

    Citrine

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Chicken ArcLight S174

    Plasmid

    #53567

    Purpose

    Genetically encoded voltage sensor ArcLight with improved kinetics

    Depositor

    Vincent Pieribone

    Article

    Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

    Insert

    Chicken ArcLight-S174
    

    Expression

    Mammalian

    Mutation

    Gg-VSP contains an R153Q mutation and an amino ac…

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3xFLAG-CMV TIP60 390-513

    Plasmid

    #78797

    Purpose

    To overexpress TIP60 390-513 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    TIP60 (KAT5 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    aa390-513

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pETM33_Nsp3c_SUD-N

    Plasmid

    #156469

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM33_Nsp3c_SUD-M

    Plasmid

    #156470

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

    Plasmid

    #128278

    Purpose

    Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

    Depositor

    Anna Sokac

    Article

    Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

    Insert

    twinstar (tsr) (tsr Fly)
    

    Tags

    6X Histidine tag

    Expression

    Bacterial

    Mutation

    Met1 changed to Pro1; Cys77 silent basepair mutat…

    Promoter

    T7

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3 Flag MFF iso5 S155A

    Plasmid

    #74381

    Purpose

    Flag tagged human S155A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET21a-MKP7_K55R_153

    Plasmid

    #37794

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 55 to Arginine and deleted amino a…

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only