We narrowed to 59,942 results for: %s

1. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Available Since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMV-OptoSTIM1(Cry2(D387A))

   Plasmid

   #70160

   Purpose

   Light-insensitive mutant of OptoSTIM1

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. eTd-CBEa

   Plasmid

   #196603

   Purpose

   Mammalian expression of eTd-CBEa

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

   Plasmid

   #107700

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   QuasAr3-P2A-CheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human synapsin I

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXN2KS-Dsup

   Plasmid

   #90019

   Purpose

   To establish stable line expressing Dsup in constitutive manner

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti HsATP13A2 D962N

   Plasmid

   #171821

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   WT, D508N

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. K9L mutant of H3K9me3 biosensor

   Plasmid

   #120807

   Purpose

   FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
   

   Expression

   Mammalian

   Mutation

   lysine 9 is mutated to leucine 9 on histone H3

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

   Plasmid

   #216320

   Purpose

   Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Luciferase + splice donor site
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVtrunc Affimer6-sfCherry2ΔN12

    Plasmid

    #231551

    Purpose

    Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Affimer6
    

    Tags

    sfCherry2

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CPSF4-1

    Plasmid

    #155478

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF4 (CPSF4 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-N1 GBP (GBP-mCherry)

    Plasmid

    #162879

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HA-CDK2

    Plasmid

    #172613

    Purpose

    Expresses HA-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166010

    Purpose

    mammalian expression of SARS-CoV-2 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S )
    

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF CAG BFP2 IRES neo

    Plasmid

    #108175

    Purpose

    Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Blue fluorescent protein (BFP2)
    

    Use

    Lentiviral; Fluorescent protein

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET20b-mPTER

    Plasmid

    #222189

    Purpose

    Bacterial expression of mouse PTER protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCB306-GFP-mito

    Plasmid

    #229232

    Purpose

    Expresses GFP localized to mitochondria

    Depositor

    Suzanne Pfeffer

    Article

    Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

    Insert

    EGFP-MAOA (MAOA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EXOSC10

    Plasmid

    #155589

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    EXOSC10 (EXOSC10 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-N1-SAV1

    Plasmid

    #166476

    Purpose

    Expresses fusion of mEGFP and SAV1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, SAV1 (SAV1 Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. YA1532: pAAV_ CAG-FLEX-paQuasAr3

    Plasmid

    #107702

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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