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pKP381

Plasmid

#167592

Purpose

Express TonB H20A protein

Depositor

Kathleen Postle

Article

Larsen et al J Bacteriol. 2007 Apr;189(7):2825-33. do

Insert

tonB H20A

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1

Plasmid

#160448

Purpose

BioBrick mammalian-codon-optimized mRFP1

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-dsRed

Plasmid

#160449

Purpose

BioBrick mammalian-codon-optimized dsRed

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, dsRed

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1302

Plasmid

#160817

Purpose

Expression of FepA protein

Depositor

Kathleen Postle

Article

Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

Insert

fepA (fepA Escherichia coli)

Expression

Bacterial

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAC042

Plasmid

#156344

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNK041p

Plasmid

#156331

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-tagRFP-IMBB2.4-pUC57-mini

Plasmid

#149343

Purpose

expresses tagRFP fluorescent protein in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

tagRFP

Expression

Bacterial

Promoter

rhamnose

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pExcisionase-AntiDsRed

Plasmid

#139675

Purpose

Cassette for testing phiC31 Excisionase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed phiC31 Excisionase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGD252

Plasmid

#132373

Purpose

contains the sequence of TSapphire for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-TSapphire

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD254

Plasmid

#132375

Purpose

contains the sequence of mOrange for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mOrange

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD141

Plasmid

#132379

Purpose

contains the sequence of mKate2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mKate2

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD318

Plasmid

#129735

Purpose

Contains a myristoylation sequence in the pGGC000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

Myristoylation sequence (MGRRKRKPK-)

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCC2

Plasmid

#128283

Purpose

asymmetric plasmid partitioning E. coli - negative control

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, _parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB849

Plasmid

#128298

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - GlySer-cfp-parB

Expression

Bacterial

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yeVenus-T2A-mTurquoise2

Plasmid

#118440

Purpose

Produces equimolar expression of yeVenus and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yeVenus-T2A-mTurquoise2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tagRFP-T2A-mTurquoise2

Plasmid

#118437

Purpose

Produces equimolar expression of tagRFP and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tagRFP-T2A-mTurquoise2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mTurquoise2-T2A-mcherry

Plasmid

#118433

Purpose

Produces equimolar expression of mTq2 and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mTurquoise2-T2A-mcherry

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Clover-T2A-mTurquoise2

Plasmid

#118425

Purpose

Produces equimolar expression of Clover and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

Clover-T2A-mTq2

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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