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PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64T BMPR (DM#136)

Plasmid

#15068

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pAJ13

Plasmid

#14915

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMV2 BMPR (DM#138)

Plasmid

#15069

Depositor

Douglas Melton

Article

Graff et al Cell. 1994 Oct 7. 79(1):169-79.

Insert

BMP receptor (bmpr1a Frog)

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pCL0956

Plasmid

#252420

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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V12

Plasmid

#253336

Purpose

Fully genetically encoded protein tag for CryoET labelling

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

V12

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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Delta6

Plasmid

#253337

Purpose

Fully genetically encoded protein tag for CryoET labelling

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

Delta6

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0943

Plasmid

#252414

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

iFP2.0-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0941

Plasmid

#252413

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

TagBFP-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0919

Plasmid

#252412

Purpose

Auto-Inhibited TVMV protease for the RAPID-release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

TVMV-AI

Tags

mCherry-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0639

Plasmid

#252410

Purpose

NASP tagged on the N-terminus with mCherry.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

sNASP (NASP Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1011

Plasmid

#252432

Purpose

RbAp46 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

RbAp46 (RBBP7 Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK125

Plasmid

#245147

Purpose

pJAK081 carrying Pcwp2-mScarlet-I

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mScarlet-I

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK201

Plasmid

#245149

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 1 (AAGTCCTCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK202

Plasmid

#245150

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 2 (TCTTGACCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK203

Plasmid

#245151

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 3 (AACAACACC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK204

Plasmid

#245152

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 4 (AACAGGTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK205

Plasmid

#245153

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 5 (ACCGATTAG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK206

Plasmid

#245154

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 6 (CACTTATGC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK207

Plasmid

#245155

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 7 (CCTCCAACT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge