We narrowed to 13,644 results for: Mpl

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pETM11-6xHis-TEV-hORF1p

Plasmid

#202589

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

Depositor

Kathleen Burns, Liam Holt

Article

Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

Insert

ORF1p

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Plasmid

#217510

Purpose

KD Recombinase dependent conditional smGdP-10XV5 cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Tags

smGdP-10XV5

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pF146 pSOD1WTAcGFP1

Plasmid

#26407

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC78

Plasmid

#62339

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot8-D40AE42A_U

Plasmid

#147647

Purpose

Mammalian Expression of HsNot8-D40AE42A

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8-D40AE42A (CNOT8 Human)

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-JUN

Plasmid

#79512

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

JUN (JUN Human)

Use

Multisite gateway entry clone

Tags

HA Tag

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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OTR untagged

Plasmid

#208913

Purpose

encodes the human oxytocin receptor, without any tags

Depositor

Christian Gruber

Article

Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

Insert

OXTR (OXTR Human)

Expression

Mammalian

Mutation

TM5: 5x56, A218, Threonin instead of Alanin

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTmycDICER

Plasmid

#19873

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

dicer 1, ribonuclease type III (DICER1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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CLuc-FKBP

Plasmid

#31184

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark3

Plasmid

#233145

Purpose

Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark3

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-FOS

Plasmid

#79511

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

FOS (FOS Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp10_SARS-CoV-2

Plasmid

#167851

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA4.TO-ORF4-2xCSTREP

Plasmid

#136169

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF4

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF4

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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