We narrowed to 6,930 results for: Proc;
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Plasmid#169965PurposepDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only
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P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
Tags3xHAExpressionYeastMutation∆RPromoterpATG8Available SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-17b_Unc104-KIF5B-HALO
Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
TagsHALO-tag, 6xHISExpressionBacterialPromoterT7Available SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SERPINA5_Serpin
Plasmid#110034PurposeProtein expression and purification of SERPINA5_SerpinDepositorInsertSERPINA5_Serpin (SERPINA5 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC365
Plasmid#91214Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A
Plasmid#140451PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN1779
Plasmid#127165PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)DepositorInsertpGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
UseSynthetic BiologyAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
TagsH6ExpressionBacterialMutationNonePromoterT7Available SinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRRP12-EBMmut_K
Plasmid#146767PurposeMammalian Expression of HsRRP12-EBMmutDepositorInsertHsRRP12-EBMmut (RRP12 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC366
Plasmid#91215Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNA repair library with 1,573 sgRNAs
Pooled Library#177663PurposeCRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processesDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout Library with UMIs
Pooled Library#221409PurposegRNA library to test the impact of transporters on biological processesDepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
DNA repair library with 336 sgRNAs
Pooled Library#177664PurposeCRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processesDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FZ-NbPDS
Plasmid#184268PurposepAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.DepositorInsertCPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
ExpressionPlantMutationThe protease processing site between MP and L-CP …PromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRExpressionMammalianMutationDR1 A>TPromoterU6Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IBU-pSu9-HaloTag7-SNAP
Plasmid#217319PurposeExpresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.DepositorInsertNeurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
UseRetroviralTagsHaloTag and SNAPPromoterCMVAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only