We narrowed to 15,981 results for: LEA

1. pcDNA3 TFAM-mClover

   Plasmid

   #129574

   Purpose

   Expression of mClover fused TFAM

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   TFAM (TFAM Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB_CRF1.0

   Plasmid

   #208655

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-Gal3

   Plasmid

   #85662

   Purpose

   Fluorescent reporter for lysosomal damage

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   LGALS3 (LGALS3 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL-XPG

   Plasmid

   #39215

   Depositor

   Aziz Sancar

   Article

   Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

   Insert

   XPG (ERCC5 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Note that XPG starts at amino acid 5

   Promoter

   Ptac

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 TFAM-mScarlet

   Plasmid

   #129573

   Purpose

   Expression of mScarlet fused TFAM

   Depositor

   Stephen Tait

   Article

   Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

   Insert

   TFAM (TFAM Human)
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_AU1.C.HA_mCherry-NLS

   Plasmid

   #178209

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.C.HA and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLXEBR-pTF-cKit

   Plasmid

   #114293

   Purpose

   TET-inducible expression of c-Kit

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   cKit (Kit Mouse)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-6P1-hPXR-LBD

    Plasmid

    #67769

    Purpose

    Expression GST-hPXR-LBD in bacteria

    Depositor

    Sridhar Mani

    Article

    Li et al J Biol Chem. 2013 May 10;288(19):13655-6

    Insert

    human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains amino acids 107 to 434 of human PXR

    Promoter

    Tac

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-fDIO-ArgiNLS-mKate2

    Plasmid

    #220607

    Purpose

    Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-fDIO-ArgiNLS-oScarlet

    Plasmid

    #220606

    Purpose

    Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

    Plasmid

    #187582

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF7 (SRSF7 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

    Plasmid

    #168998

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Unspecified

    Tags

    EGFP and mRFP1

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLHCX-XBP1 mNeonGreen NLS

    Plasmid

    #115971

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Retroviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-FLAG-HA-(N)-CDK12

    Plasmid

    #231750

    Purpose

    Expression in Flp-IN human cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    CDK12 (CDK12 Human)
    

    Tags

    Flag-HA-3C

    Expression

    Mammalian

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMGF196

    Plasmid

    #97005

    Purpose

    Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV delta 5

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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