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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 6841 - 6860 of 14067 results

  6. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Publication
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122243-plasmid-map-sequence-id-239188

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  8. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

    Plasmid
    #178868
    Purpose
    Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA
    Depositor
    Shinya Tsukiji
    Publication
    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178868-plasmid-map-sequence-id-355800

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  9. pCEP4-myc-S (Wuhan variant)

    Plasmid
    #169846
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Publication
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169846-plasmid-map-sequence-id-329062

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  12. pCI-Flag-NEDD4L∆WW2

    Plasmid
    #166708
    Purpose
    expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W453A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 453 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166708-plasmid-map-sequence-id-329114

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  13. pCI-Flag-NEDD4L∆WW3

    Plasmid
    #166709
    Purpose
    expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells
    Depositor
    Wesley Sundquist
    Publication
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEDD4L W525A
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 525 to alanine
    Promoter
    CMV promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166709-plasmid-map-sequence-id-329115

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  17. Venus-30aa-Noxa-pEGFP-C1

    Plasmid
    #166762
    Purpose
    To increase the length of the linker between Venus and Noxa proteins
    Depositor
    David Andrews
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Noxa (PMAIP1 Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166762-plasmid-map-sequence-id-328844

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Showing: 6841 - 6860 of 14067 results