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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,343 results for: SHE

Showing: 6841 - 6860 of 18343 results

  1. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  2. pLV-Enh eGFP Reporter-muEDC-Int3

    Plasmid
    #59288
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mFlg int-Intron 1
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

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  5. CSNK1G2

    Plasmid
    #39150
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G2 (CSNK1G2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

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  7. pIRES-Neo-HA-cdc25C1

    Plasmid
    #40966
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

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  9. SLC9A3R2

    Plasmid
    #38948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  13. pUASP Flag Rab32 WT

    Plasmid
    #44833
    Depositor
    Matthew Scott
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. ENOSF1

    Plasmid
    #39104
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENOSF1 (ENOSF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pIRES-Neo-HA-cdc25C5

    Plasmid
    #40967
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Available Since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

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  17. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  18. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  19. pUC57-sgDedd-1

    Plasmid
    #74435
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 5
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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Showing: 6841 - 6860 of 18343 results