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TDP-43_CTD_M339A

Plasmid

#248886

Purpose

Edit Expresses 6xHis-tagged C-terminal domain of TDP-43 with M339A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M339A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M339A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_L340A

Plasmid

#248887

Purpose

Edit Expresses 6xHis-tagged C-terminal domain of TDP-43 with L340A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_L340A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed L340A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_S342A

Plasmid

#248888

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S342A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S342A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S342A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M336A

Plasmid

#248884

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M336A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M336A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M336A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_S333A

Plasmid

#248882

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S333A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S333A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S333A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_W334A

Plasmid

#248883

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with W334A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_W334A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed W334A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-MLLn-CAGEn-NES (Nrdj1)

Plasmid

#241412

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

MLLn-CAGEn-NES (Nrdj1) (KMT2A Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-MLLn-CAGEn-NES (Npu)

Plasmid

#241411

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

MLLn-CAGEn-NES (Npu) (KMT2A Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AMLn-CAGEn-NES (Nrdj1)

Plasmid

#241408

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Figure 2 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

AMLn-CAGEn-NES (Nrdj1) (RUNX1 Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-NGN3-linker-3xFLAG-Dendra2-HA

Plasmid

#234887

Purpose

Expresses human NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags

Depositor

Nancy Papalopulu

Article

Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

Insert

NGN3-3xFLAG-Dendra2-HA (NEUROG3 Human)

Tags

3xFLAG, Dendra2 and HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AMLn-CAGEn-NES (Npu)

Plasmid

#241407

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

AMLn-CAGEn-NES (Npu) (RUNX1 Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pBC001 v3

Plasmid

#161711

Purpose

CMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPRE

Depositor

Nozomu Yachie

Article

Nakayama et al Sci Immunol. 2024 May 24;9(95):eade3814.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CAG_G-PTEN

Plasmid

#227436

Purpose

Expresses the G-PTEN sensor under a CAG promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)

Tags

mEGFP, and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Expression

Mammalian

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCA528 IST1 L328D, L355A (1-366)

Plasmid

#193040

Purpose

Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

L328D, L355A

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 V18D-OSF

Plasmid

#193042

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

V18D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG CAPN7 L61D-OSF

Plasmid

#193043

Purpose

Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

CAPN7 (CAPN7 Human)

Tags

OSF (One-Strep-FLAG)

Expression

Mammalian

Mutation

L61D

Promoter

Chicken Beta-actin

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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