We narrowed to 4,653 results for: luciferase

1. pcDNA5/FRT/TO-GAlphasS-RLuc8

   Plasmid

   #140980

   Purpose

   Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasS-RLuc8
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
   

   Tags

   NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATG-2460

   Plasmid

   #108713

   Purpose

   EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt-copGFP fusion
   

   Use

   Lentiviral

   Tags

   CBR2opt fused to copGFP

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   elongation factor-1

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

   Plasmid

   #123363

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   EGFP-NLS
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pscAAV-CAG-RLuc

   Plasmid

   #83280

   Purpose

   recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

   Insert

   Renilla Luciferase
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-FLuc

   Plasmid

   #83281

   Purpose

   recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119946

   Purpose

   Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Promoter

   TetCMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNOC_hfnCas12a_CRATES

   Plasmid

   #176251

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo-RL-ZEB1

   Plasmid

   #35535

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB1 (ZEB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATG-3925 (CBG2)

    Plasmid

    #167839

    Purpose

    Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

    Depositor

    Lance Encell

    Article

    Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

    Insert

    Click Beetle Green 2
    

    Tags

    6-His

    Expression

    Bacterial and Mammalian

    Mutation

    Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

    Promoter

    CMV, T7

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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