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We narrowed to 46,691 results for: cha

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Showing: 6861 - 6880 of 46691 results

  1. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (AAV PHP.eB)

    Viral Prep
    #163505-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (#163505). In addition to the viral particles, you will also receive purified AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) plasmid DNA. AAV vector for strong and rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core sequence has 100% sequence conservation between mouse and human. Developed especially for rapid viral labeling in human and NHP organotypic brain slice culture. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Promoter
    minBetaGlobin
    Tags
    SYFP2
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only

  3. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Expression
    Mammalian
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

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  5. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  7. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  10. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

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  12. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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Showing: 6861 - 6880 of 46691 results