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Showing: 6861 - 6880 of 18384 results

  2. VWF-pHluorin-mRFP

    Plasmid
    #234367
    Purpose
    Von Willebrand factor fused in tandem to superecliptic pHluorin and inserted into pmRFP1-N1 vector from Addgene (Plasmid No: 54635)/Monitoring of WPB pH, Visualization of WPB exocytosis
    Depositor
    Volker Gerke
    Article
    Terglane et al PLoS One. 2022 Jun 29;17(6):e0270299. do
    Insert
    Von Willebrand factor (VWF Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    234367-plasmid-map-sequence-id-469472

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  3. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  5. pJAT62

    Plasmid
    #204301
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204301-plasmid-map-sequence-id-405845

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  6. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177434-plasmid-map-sequence-id-348465

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  9. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

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  10. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

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  11. pAAV hSyn1 bPAC cMyc T2A tDimer

    Plasmid
    #85397
    Purpose
    humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Promoter
    human synapsin-1 and ribosomal skip sequence T2A
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    85397-plasmid-map-sequence-id-201306

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  12. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-F
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

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  14. JARID1BA

    Plasmid
    #74648
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pJH131

    Plasmid
    #48260
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    GAL10/GAL1 bidirectional
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    48260-plasmid-map-sequence-id-69695

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Showing: 6861 - 6880 of 18384 results