We narrowed to 12,189 results for: LEA
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Plasmid#68996Purposefor making transgenic fishDepositorAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only
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pFSynW Substance P-pHluorin IRES mRuby3
Plasmid#195699PurposeLentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HiFi SpCas9
Plasmid#207362PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HiFi SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196883PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-FKBP-PRPF38A
Plasmid#187968Purposeexpresses PRPF38A with N-terminal tags RFP and FKBPDepositorInsertPrp38A (PRPF38A Synthetic, Human)
UseCmv promoterTagsmRFP-FKBPExpressionMammalianPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-CAG-mRFP-PCNA pc2729
Plasmid#166040PurposeExpresses mRFP tagged human PCNA in mammalian cells.DepositorAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1b in pT3TS-Dest
Plasmid#194295PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LACDepositorPublicationInsertcavin1b (cavin1b Zebrafish)
UseIn vitro transcription of mrnaAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc AspCas12a
Plasmid#182122PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc AspCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mcGAS-LL/RK-NLS
Plasmid#185457PurposeInducible nuclear cGASDepositorPublicationInsertcGAS
UseLentiviralTagsFLAG and SV40 NLSExpressionMammalianMutationL157R, L159KAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)
Plasmid#185706PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLSDepositorInsertCas9
Tags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRExpressionBacterialMutationD917A (nuclease-deactivating)Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PB1-MontiRed-LOV2dark
Plasmid#178874PurposeMammalian expression of PB1-MontiRed-LOV2darkDepositorInsertPB1-MontiRed-LOV2dark
ExpressionMammalianMutationLOV2: C450A, L514K, G528A, L531E, N538EPromoterCAGAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-Dendra2-Puro
Plasmid#169217PurposeTargeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked Dendra2-P2A-Puro
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen
Plasmid#174715PurposeStable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutationsDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332, E151R, R154EAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-SELF
Plasmid#158770PurposeExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.DepositorInsertSynaptophysin 1-NS3/4a (Syt1 Mouse)
UseLentiviralMutationFlexible linkers, ADVVCC/QMSY cleavage siteAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only