We narrowed to 15,981 results for: LEA

1. pTarget

   Plasmid

   #214052

   Purpose

   Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Target sequence
   

   Use

   Target rna plasmid

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHochTypeIII

   Plasmid

   #214039

   Purpose

   Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Cas10
   

   Use

   CRISPR

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF.delmFasL.CMV.GFP

   Plasmid

   #17620

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Insert

   delFasL (Fasl Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Noncleavable mouse delFasL . Deleted the exon 2 r…

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRN120

   Plasmid

   #160696

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Inserts

   CmYLCV::Luc+::AtHSP
   Nos::WUS2::PinII
   AtUbi10::STM::35S
   

   Use

   Luciferase; Gemini viral replicon, plant developm…

   Promoter

   AtUbi10, CmYLCV, and Nos

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn Con/Fon EYFP

   Plasmid

   #55650

   Purpose

   Cre-on/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   eYFP with introns
   

   Use

   AAV; Cre on/flp on eyfp

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

   Plasmid

   #182423

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-eythropoietin (EPO Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TVBB N-term-mTurquoise2

   Plasmid

   #169222

   Purpose

   Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

   Plasmid

   #187579

   Purpose

   Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PSPC1 (PSPC1 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK6-HA-TAK1

    Plasmid

    #44160

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. EGFP-HsNRF2 (NFE2L2)

    Plasmid

    #194303

    Purpose

    Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

    Plasmid

    #187585

    Purpose

    Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NPAT (NPAT Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ shSTING-Mmu

    Plasmid

    #127697

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse STING gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shSTING Mmu (Sting1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 7TFP CDH1 mutant reporter

    Plasmid

    #91703

    Purpose

    lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    CDH1 mutant promoter (CDH1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    mutant E-boxes

    Promoter

    CDH1 mutant

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

    Plasmid

    #235091

    Purpose

    SRSF3 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TVBB C-term-mScarlet

    Plasmid

    #169219

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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