We narrowed to 7,032 results for: SIM;
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Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
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pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
ExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS
Plasmid#140013PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry PGK-1 in pcDNA3.1
Plasmid#64214PurposemCherry fused between Cry2 and PGK-1 to study cell migrationDepositorTagsmcherryExpressionMammalianPromoterCMVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/A
Plasmid#48792PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygroDepositorInsertYFP-synhDAT-S/A (SLC6A3 Human)
TagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-synDAT-S/D
Plasmid#48793PurposeExpresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygroDepositorInsertYFP-synhDAT-S/D (SLC6A3 Human)
TagsYFPExpressionMammalianMutationSer2, Ser4, Ser7, Ser12, and Ser13 mutated simult…Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT A41AA C55A sfGFP 150TAG
Plasmid#154770Purposeplasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, G1 PylT with A41AA and C5…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-moxBFP
Plasmid#242410PurposeSelf-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (vaccinia virus E3, Toscana virus NSs, Theiler’s virus L*) and moxBFP (in place of inflammatory signaling inhibitors).DepositorInsertsmScarlet3
IRES-E3-NSs-L*-EGFP
IRES-moxBFP
UseMouse Targeting; Self-amplifying rnaExpressionMammalianAvailable SinceSept. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα
Plasmid#242411PurposeSelf-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).DepositorInsertsmScarlet3
IRES-E3-NSs-L*-EGFP
IRES-srIκBα
UseMouse Targeting; Self-amplifying rnaExpressionMammalianMutationserines 32 and 36 to alaninesAvailable SinceSept. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-EGFP-IRES-E3-NSs-L*
Plasmid#242409PurposeSelf-amplifying RNA (saRNA) E3-NSs-L* construct; co-expresses vaccinia virus E3, Toscana virus NSs, and Theiler’s virus L*; more comprehensive inhibition of dsRNA-sensing pathways than plasmid 242408.DepositorInsertsmScarlet3
IRES-EGFP
IRES-E3-NSs-L*
UseMouse Targeting; Self-amplifying rnaExpressionMammalianAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-A
Plasmid#221713PurposeExpression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-A (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationD683A in active sitePromotersimian CMV IE94Available SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGMutationF666Y in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2E-D
Plasmid#221721PurposeExpression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2E-D (AGO2 Human)
UseFish expressionTags3xFLAGMutationE637D in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2D-A
Plasmid#221720PurposeExpression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2D-A (AGO2 Human)
UseFish expressionTags3xFLAGMutationD669A in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2Y-F
Plasmid#221715PurposeExpression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2Y-F (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationY680F in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drAgo2D-E
Plasmid#221714PurposeExpression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInsertdrAgo2D-E (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationD651E in active sitePromotersimian CMV IE94Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA
Plasmid#174895Purposeamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR328-3p
Plasmid#153319PurposeExpresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR328-3p sponge
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-anti-MIR211-5p
Plasmid#153318PurposeExpresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.DepositorInsertzsGreen with MIR211-5p sponge
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
ExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only