We narrowed to 14,321 results for: cas9

Showing: 6881 - 6900 of 14321 results

Results per page:

Sort results by:

AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Publication

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222857

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CTG)6

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCTG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222859

Purpose

This plasmid codes for the Slug-KH nickase with a sg(AGC)6

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgAGC

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222858

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CAG)6

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCAG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222860

Purpose

This plasmid codes for the Slug-KH nickase with a sg(GCA)6

Depositor

Vincent Dion

Publication

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgGCA

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C114m

Plasmid

#121013

Purpose

MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

dCas9 with no bbsI or BsaI sites

Use

Synthetic Biology

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

Plasmid

#121174

Purpose

Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro

Use

CRISPR

Expression

Mammalian

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ZNF219_1

Plasmid

#72368

Purpose

Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF219 (ZNF219 Human)

Use

CRISPR

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Publication

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Reckleen_3plus_sgRNA_ptet_pos4

Plasmid

#233459

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Publication

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.

Use

CRISPR

Available since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pXZX353

Plasmid

#169457

Purpose

MATα (alpha) fragment in pTOPO vector

Depositor

Jef Boeke

Publication

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

MatA

Expression

Bacterial

Promoter

lac

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-CR7T-gRNA2.1-nlsBFP

Plasmid

#170515

Purpose

gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

Depositor

Chun Han

Publication

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

CR7T

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Publication

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM35171

Plasmid

#153222

Purpose

Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, BAR gene, Ocs terminator

Use

Synthetic Biology

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1164

Plasmid

#111100

Purpose

Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB4778

Plasmid

#106125

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCfB6631

Plasmid

#106161

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge