We narrowed to 17,802 results for: erg

1. pX459-HypaCas9-mR2-H2BC11_sgRNA

   Plasmid

   #183881

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-mR2-MYH10_sgRNA

   Plasmid

   #183886

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

   Plasmid

   #146121

   Purpose

   Insect Expression of DmEDC3_1-101

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmEDC3_1-101 (Edc3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A456G and three mutations N16…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

   Plasmid

   #146122

   Purpose

   Insect Expression of DmEDC3_1-338

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   DmEDC3_1-338 (Edc3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A456G and three mutations N16…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPLN-hFluc-DM-HA-GFP11-KDEL

   Plasmid

   #177723

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Use

   Tags

   GFP11, HA, KDEL, and Prolactin Signal Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p406 – TDH3-ER-FlucDM-GFP- Ura

   Plasmid

   #177732

   Purpose

   DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   FlucDM
   

   Use

   Tags

   HA, HDEL, KAR2 Signal Sequence, and eGFP

   Expression

   Bacterial and Yeast

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOME0233M

   Plasmid

   #178415

   Purpose

   Reverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   Reverse complement of pOME0134 upstream MIR-SYN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOME0232M

   Plasmid

   #178414

   Purpose

   REVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV Reverse complement of downstream MIR-SYN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.U6gT28.4.hSyn.H2B.RFP

   Plasmid

   #170387

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B RFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human Synapsin

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.U6gT28.3.hSyn.H2B.RFP

    Plasmid

    #170370

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.U6gT28.13.hSyn.H2B.RFP

    Plasmid

    #170372

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV.U6gT28.4.EFS-NS.H2B-RFP

    Plasmid

    #170365

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV.U6gT28.3.EFS-NS.H2B-RFP

    Plasmid

    #170364

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE30-HypocratesCS

    Plasmid

    #161957

    Purpose

    Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

    Depositor

    Dmitry Bilan

    Article

    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

    Insert

    HypocratesCS
    

    Use

    Tags

    6xHIs tag

    Expression

    Bacterial

    Mutation

    changed cysteine 355 to serine

    Promoter

    T5

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKII-GCaMP6f-4x128-mAGNET

    Plasmid

    #166023

    Purpose

    Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    GCaMP6f with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKII-mRuby2-4x128-mAGNET

    Plasmid

    #166021

    Purpose

    Expresses mRuby in CaMKII+ cells with low miRNA-128 expression

    Depositor

    Xue Han

    Article

    Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

    Insert

    mRuby2 with 4 miR-128 binding sites
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-10xPP7-Qb model based

    Plasmid

    #158203

    Purpose

    Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7gRNA-smyhc1_977

    Plasmid

    #140867

    Purpose

    sgRNA synthesis vector for smyhc1_977 (zebrafish slow myosin heavy chain 1).

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    zebrafish smyhc1_977 sgRNA for in vitro transcription (smyhc1 Zebrafish, Synthetic)
    

    Use

    In vitro transcription of sgrnas

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7gRNA-ttn.1_C3

    Plasmid

    #140866

    Purpose

    sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    zebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Zebrafish, Synthetic)
    

    Use

    In vitro transcription of sgrnas

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge