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pJZC638

Plasmid

#62283

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been deactivated by mutatio…

Promoter

pAdh and pGal10

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
p6618 MSCV-IP N-HAonly WHSC2

Plasmid

#35014

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WHSC2 (NELFA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pQE9-His-p97(QQ)

Plasmid

#14667

Depositor

Graham Warren

Article

Ye et al Nature. 2001 Dec 6. 414(6864):652-6.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

E305/578Q

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pDONR223_MAPK7_WT

Plasmid

#82925

Purpose

Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_002749.3 (MAPK7 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-memGFP-P2A

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E243 Hs.RASGRP1

Plasmid

#70527

Purpose

Gateway ORF clone of human RASGRP1 [NM_005739.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

RASGRP1 (RASGRP1 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-AAK1

Plasmid

#23844

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AAK1 (AAK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAG304GAL-ccdB

Plasmid

#14135

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pMCSG7

Plasmid

#119925

Purpose

Nme2Cas9 bacterial expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS, Nucleoplasmin NLS, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF4_WT

Plasmid

#82190

Purpose

Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF4 (ATF4 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKSS

Plasmid

#39272

Depositor

Peter Kast

Article

Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

Type

Empty backbone

Use

Positive-selection cloning vector

Expression

Bacterial

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only

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CMPE-ePPEplus

Plasmid

#205242

Purpose

For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-FURIN

Plasmid

#158386

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

furin, paired basic amino acid cleaving enzyme (FURIN Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLD-puro-Cc-TARDBP-WT_VA

Plasmid

#141327

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-WT (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST-Constitutively Active AMPK

Plasmid

#139843

Purpose

Lentiviral expression of GST-tagged Constitutively Active rat AMPK

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-OGDH_wobble

Plasmid

#110938

Purpose

OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

OGDH wobble cDNA (OGDH Human)

Use

Lentiviral

Mutation

15 silent wobble mutations corresponding to three…

Promoter

SV40

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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