We narrowed to 7,645 results for: tet

1. pAAV-mGFAP-Lck-PinkFlamindo

   Plasmid

   #228394

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   Pink Flamindo
   

   Use

   AAV

   Promoter

   mGFAP

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINTO-NFH::hSNRNP70

   Plasmid

   #65926

   Purpose

   Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Two silent mutations

   Promoter

   CMV/TO and CMV/TetO2 (T-REx)

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-UTX-F-MT2

   Plasmid

   #40619

   Purpose

   Expression of enzyme-dead UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTX (KDM6A Human)
   

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   changed Histidine 1146 and Glutamic Acid 1148 to …

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-Nsp16

   Plasmid

   #157723

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16 (ORF1ab SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEM-Cas9HF1

   Plasmid

   #89961

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9HF1
   

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-HRASG12V

   Plasmid

   #216509

   Purpose

   Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   HRAS (HRAS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC34

    Plasmid

    #62331

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ134-STU-As

    Plasmid

    #138103

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVTRE3_Clover

    Plasmid

    #135179

    Purpose

    AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid

    #157933

    Purpose

    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

    Insert

    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    

    Tags

    sfGFP & TwinStrep

    Expression

    Mammalian

    Mutation

    E190D

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 3xFlag IFIT2

    Plasmid

    #53555

    Purpose

    mammalian expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ131-STU-Lb

    Plasmid

    #138096

    Purpose

    Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Puro_siKO

    Plasmid

    #86696

    Purpose

    Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    H1 TO

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ134-STU-Lb

    Plasmid

    #138105

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge