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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,179 results for: Pol

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Showing: 6961 - 6980 of 10179 results

  2. pBactin-mNeonGreen-CI

    Plasmid
    #196850
    Purpose
    Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196850-plasmid-map-sequence-id-391800

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  4. HiFi iFE

    Plasmid
    #190141
    Purpose
    Expresses HiFiCas9-POLB (HiFi iFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9-POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190141-plasmid-map-sequence-id-377886

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  6. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162678-plasmid-map-sequence-id-321524

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  7. pGBW-m4134347

    Plasmid
    #152890
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152890-plasmid-map-sequence-id-487616

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  9. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

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  16. psicheck-2-Casp8

    Plasmid
    #48172
    Purpose
    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element
    Depositor
    Vamsi Mootha
    Publication
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5" UTR of CASP8 (CASP8 Human)
    Use
    Luciferase
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. ACSM2B

    Plasmid
    #39167
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACSM2B (ACSM2B Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Mutation
    Actually the mitochondrial form, GI:58082049, wit…
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6961 - 6980 of 10179 results