We narrowed to 14,099 results for: cas9

1. pLV-U6-UL29-egfp-U3-UL8

   Plasmid

   #166694

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
   

   Use

   Lentiviral

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLsgRNA-AtU3d

   Plasmid

   #66200

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3d

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-egfp-U3-Osp.gRNA

   Plasmid

   #166034

   Purpose

   Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUFlip-floxed2A-mRFP-; HE1A:BFP -0

   Plasmid

   #180007

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUFlip-floxed2A-mRFP-; gcry1:BFP -2

   Plasmid

   #173888

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-eNme2-T.2-ABE8e

   Plasmid

   #185669

   Purpose

   Expresses eNme2-T.2-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUFlip-floxed2A-mRFP-; HE1A:BFP -1

   Plasmid

   #180008

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYLsgRNA-AtU3d/LacZ

   Plasmid

   #66201

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3d

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-sgRNA-lib 2.0

    Plasmid

    #89638

    Purpose

    sgRNA expression in mammalian cells after Gateway cloning

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-MGAT1-KO

    Plasmid

    #80009

    Purpose

    gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    MGAT1 (MGAT1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330 PLIN2

    Plasmid

    #202196

    Purpose

    pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

    Plasmid

    #173887

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUS2-SH231

    Plasmid

    #115150

    Purpose

    Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Dual SH231 gRNAs
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYLsgRNA-OsU6c

    Plasmid

    #66197

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU6c

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. BLADE

    Plasmid

    #134912

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system

    Depositor

    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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