We narrowed to 2,952 results for: BFP

1. Str-KDEL_ST-SBP-tagBFP

   Plasmid

   #65266

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

   Plasmid

   #105034

   Purpose

   Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Ptpn23 (Ptpn23 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

   Plasmid

   #200475

   Purpose

   Lentiviral vector expressing gRNA targeting GFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA EBFP2

   Plasmid

   #167905

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

   Plasmid

   #208548

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(13) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

   Plasmid

   #208547

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(12) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

   Plasmid

   #105039

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98972

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPRISM-Stop-gcry-TagBFP

    Plasmid

    #117771

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-HRI-V5-IRES-BFP

    Plasmid

    #202435

    Purpose

    Lentiviral vector expression of HRI and TagBFP

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUltra-hot-H2B-mTagBFP2

    Plasmid

    #161792

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    mCherry and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-FKBP-mTagBFP2-SOScat

    Plasmid

    #178866

    Purpose

    Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6::sgBFP-U6::sgKrasG12D-pX333-red

    Plasmid

    #149448

    Purpose

    pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    sgRNA BFP and mouse KRAS G12D
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1-rab-3P::his24::tagBFP2

    Plasmid

    #124344

    Purpose

    Nuclear Localized Pan-Neuronal tagBFP2

    Depositor

    Andrew Leifer

    Insert

    rab3, tagBFP2 (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1-rab-3P::his-24::tagBFP

    Plasmid

    #124343

    Purpose

    Nuclear Localized Pan-Neuronal tagBFP

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    rab-3, tagBFP (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. StrKDEL-IRES-mannosidase II-mTagBFP2

    Plasmid

    #165460

    Purpose

    Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

    Insert

    Partial ManII-mTag2BFP fusion (Man2a1 Mouse)
    

    Tags

    Str-KDEL and mTag2BFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge