We narrowed to 15,995 results for: LEA

1. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

   Plasmid

   #214281

   Purpose

   Express split-Rac1 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   Rac1-T17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

   Plasmid

   #214283

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   RhoA-T19N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214284

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

   Plasmid

   #214285

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #214286

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #214287

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CaMKII-somBiPOLES-mCerulean (AAV9)

   Viral Prep

   #154948-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   CaMKIIa(0.4)

   Tags

   mCerulean

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

8. CaMKII-somBiPOLES-mCerulean (AAV5)

   Viral Prep

   #154948-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   CaMKIIa(0.4)

   Tags

   mCerulean

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

9. CaMKII-somBiPOLES-mCerulean (AAV Retrograde)

   Viral Prep

   #154948-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   CaMKIIa(0.4)

   Tags

   mCerulean

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161928

    Purpose

    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine activation reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161927

    Purpose

    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine repression reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

    Plasmid

    #204810

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Expression

    Bacterial

    Available Since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #196545

    Purpose

    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

    Insert

    Surface marker and Citrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228651

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 3 portion of NS2B protease fused to NS3
    

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228635

    Purpose

    Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus A71 3C protease isoform A
    

    Tags

    His-Avi

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228634

    Purpose

    Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus Coxsackievirus A71 2A protease
    

    Tags

    His-Avi

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188743

    Purpose

    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188744

    Purpose

    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188745

    Purpose

    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    nonTATAchr21

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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