Addgene

We narrowed to 27,532 results for: NTS

Showing: 681 - 700 of 27532 results

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SRpHi2

Plasmid

#102542

Purpose

Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EGFP

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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SRpHi3

Plasmid

#102543

Purpose

Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-sepHluorin

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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SRpHi1

Plasmid

#102541

Purpose

Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EYFP

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MAVS-M142A(KaganF61)

Plasmid

#51999

Purpose

Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

HA

Expression

Mammalian

Mutation

Met 142 to Ala

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
p3XFlag-CMV7-miniMAVS(KaganE48)

Plasmid

#52019

Purpose

Expresses miniMAVS (the sequence starting from M142 of the MAVS CDS) with an N-terminal 3X flag tag

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

miniMAVS

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pReg-ROTU4

Plasmid

#190669

Purpose

Expresses an evolved rotundine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

ROTU4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-THP4

Plasmid

#190671

Purpose

Expresses an evolved tetrahydropapaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

THP4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23103

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGfOMT1-GEN4

Plasmid

#190612

Purpose

Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GfOMT-GEN4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-GLAU4

Plasmid

#190667

Purpose

Expresses an evolved glaucine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GLAU4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-PAP4

Plasmid

#190668

Purpose

Expresses an evolved papaverine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

PAP4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-NOS4

Plasmid

#190670

Purpose

Expresses an evolved noscapine biosensor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

NOS4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pBW809_pCAG-PV6-F3-mCherry-BGHpA

Plasmid

#87585

Purpose

PV6 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A-invitro(KaganE12)

Plasmid

#52016

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-G67M-invitro(KaganD100)

Plasmid

#52014

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Gly 67 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-L62M-invitro(KaganD98)

Plasmid

#52013

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Leu 62 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-E80M-invitro(KaganD99)

Plasmid

#52015

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Glu 80 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

Plasmid

#50896

Purpose

Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

L717C C723S C724V in hNKCC1 in NT17

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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