We narrowed to 4,840 results for: donor

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SacINT attP pDonor (CJT487)

Plasmid

#248227

Purpose

SacINT attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SacINT attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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BceINT attP pDonor (CJT486)

Plasmid

#248226

Purpose

BceINT attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

BceINT attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX2.5-nEGFP

Plasmid

#209287

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

Depositor

Xiaojun Lian

Publication

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-nls

Use

Knockin donor vector

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DHC1-AID-Clover donor (Hygro)

Plasmid

#158622

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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GFP hSmad4-HomDonor

Plasmid

#206044

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Publication

Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smad4 (SMAD4 Human)

Tags

EGFP

Expression

Mammalian

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMH3

Plasmid

#52528

Purpose

serves as PCR-template to generate HR donors for C-terminal GFP-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Expression

Mammalian

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Expression

Mammalian

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-N-CTCF-mAID-Halo-donor

Plasmid

#139747

Purpose

Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

Depositor

Robert Tjian

Publication

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTCF CRISPR knock-in (Ctcf Mouse)

Use

CRISPR

Tags

mAID-HaloTag

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H516 SONIC KrasG12D-IRES-luciferase donor

Plasmid

#138178

Purpose

CRISPR SONIC: Kras G12D luciferase donor plasmid.

Depositor

Wen Xue

Publication

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KrasG12D (Kras Mouse)

Use

Nhej donor

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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H517 SONIC HRASV12 donor

Plasmid

#138177

Purpose

CRISPR SONIC: HRAS G12V donor plasmid

Depositor

Wen Xue

Publication

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HRASV12 (HRAS Human)

Use

Nhej donor

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Neo)

Plasmid

#158621

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Publication

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Publication

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
Dn29 attD pDonor (CJT454)

Plasmid

#248228

Purpose

Dn29 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Dn29 attD

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Pa01 attP60 pDonor (CJT460)

Plasmid

#248225

Purpose

Pa01 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Pa01 attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-Halo donor

Plasmid

#197866

Purpose

Donor plasmid used for building MED6-Halo knock-in mESCs

Depositor

Zhe J. Liu

Publication

Dong et al bioRxiv 2022.10.26.513917

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

Halotag

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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