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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Publication

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Publication

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Expression

Bacterial

Promoter

tac

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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ilux pGEX(-)

Plasmid

#107879

Purpose

Bacterial expression of ilux for bioluminescence imaging

Depositor

Stefan Hell

Publication

Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

Insert

ilux

Expression

Bacterial

Promoter

tac

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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LeGO-V2

Plasmid

#27340

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Venus fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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