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Showing: 681 - 700 of 881 results

  1. MPC1-AA-FLAG

    Plasmid
    #228904
    Purpose
    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag
    Depositor
    Ghazaleh Ashrafi
    Article
    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do
    Insert
    MPC1 (Mpc1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K45 and K46 motif sites were both replaced with a…
    Promoter
    CMV
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228904-plasmid-map-sequence-id-460265

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  3. pBS-AMVpl-β1WT-nAchR

    Plasmid
    #97080
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97080-plasmid-map-sequence-id-189664

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  5. LUC01 (L/Sec/P)

    Plasmid
    #112144
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112144-plasmid-map-sequence-id-214867

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  6. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  10. mEos3.2trCD4o

    Plasmid
    #203751
    Purpose
    Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trCD4o (CD4 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203751-plasmid-map-sequence-id-403340

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  16. MPC1-QQ-FLAG

    Plasmid
    #228905
    Purpose
    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tag
    Depositor
    Ghazaleh Ashrafi
    Article
    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do
    Insert
    MPC1 (Mpc1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K45 and K46 motif sites were both replaced with g…
    Promoter
    CMV
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228905-plasmid-map-sequence-id-460266

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Showing: 681 - 700 of 881 results