We narrowed to 1,038 results for: transfection
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Plasmid#184081Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2(5E)-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …PromotersCMV+SP6Available SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v1
Plasmid#195138Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
ExpressionMammalianAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(G12C)
Plasmid#224284PurposeCell transfection and expression of K-Ras (G12C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(WT)
Plasmid#224283PurposeCell transfection and expression of K-RasDepositorInsertK-Ras (KRAS Human)
ExpressionMammalianAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralExpressionMammalianPromoterRetroviral 5' LTR promoterAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1
Plasmid#158775PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.DepositorInserteIF4G1
Tags3xFlagExpressionMammalianAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_Puro_Telo_v2
Plasmid#195139Purpose~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss inductionDepositorInsertTelomere (RTEL1 Human)
ExpressionMammalianAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 frt/to N-BioTAP-C-BRD4-NUT
Plasmid#171630PurposeExpresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.DepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_miniTurbo-C12orf49
Plasmid#155111Purposetransfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
TagsminiTurboExpressionMammalianPromoterCMV/TO inducible promoterAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1 +MIC
Plasmid#158776PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.DepositorInserteIF4G1 (with microexon)
Tags3xFlagExpressionMammalianAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB2_IRES_EGFP
Plasmid#223506PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_C12orf49-miniTurbo
Plasmid#155112Purposetransfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
Tags3x Flag and miniTurboExpressionMammalianPromoterCMV/TO inducible promoterAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 rtTA BirA eIF4G
Plasmid#158789PurposepcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioIDDepositorInserteIF4G1
Tags3xFlag and BirA*ExpressionMammalianPromoterminiCMVAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Gpr45-161-3xFlag
Plasmid#245993PurposeExpress 3xFlag C-terminal tagged mouse GPR45-N and GPR161-C fusion protein transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorUseLentiviralTags3xFlagExpressionMammalianMutationaa 1 to 327 of Gpr45; aa 357 to 558 of Gpr161PromoterCMVAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GSDMD_1-275
Plasmid#218230PurposeGasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 1-275Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA 3.3 N-MYC RMDN3
Plasmid#227878PurposeUsed for transfection of cells for expression of C-MYC RMDN3DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-DsRed1
Plasmid#87379PurposeRetroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).DepositorInsertTXN2 mitochondrial transit peptide (TXN2 Human)
UseRetroviralTagsDsRed1ExpressionMammalianMutationFirst 60 aa onlyPromoterRetroviral 5' LTR promoterAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 C-HA ZO1
Plasmid#227883PurposeUsed for transfection of cells for expression of N-HA ZO1DepositorAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC TB182
Plasmid#227882PurposeUsed for transfection of cells for expression of C-MYC TB182DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC PDHA1
Plasmid#227877PurposeUsed for transfection of cells for expression of C-MYC PDHA1DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only