We narrowed to 17,839 results for: She

1. pEB018

   Plasmid

   #215568

   Purpose

   Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus

   Depositor

   Robin Ohm

   Insert

   3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
   

   Use

   Fungal homologous recombination plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CAG-K-red-SPOTIT(R271F)

   Plasmid

   #191494

   Purpose

   Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   K-red-SPOTIT(R271F)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R271F in OR

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CAG-M-red-SPOTIT2(R279F)

   Plasmid

   #191495

   Purpose

   Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   M-red-SPOTIT2(R279F)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R279F in OR

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET50_G495R MDA5

   Plasmid

   #216803

   Purpose

   Expresses a single point mutant of melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G495R

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ-NUDT17-C-HA-Puro

   Plasmid

   #195182

   Purpose

   Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

   Depositor

   Stephanie Roessler

   Insert

   NUDT17 (NUDT17 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter and minimal CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7CFE1_polio_3ABC_C147R

   Plasmid

   #212985

   Purpose

   Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   cysteine 147 in 3C changed to arginine

   Promoter

   T7

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_EVA71_VP12A_C110A

   Plasmid

   #212976

   Purpose

   Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Enterovirus A71 4643 VP12A C110A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Expression

   Mutation

   changed cysteine 110 from 2A to alanine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7CFE1_polio_VP12A_C109R

   Plasmid

   #212987

   Purpose

   Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney VP12A C109R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   Cysteine 109 from 2A mutated to arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHA_SBV_NSs

   Plasmid

   #208938

   Purpose

   Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   NSs
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted 47 bp at 5' end and 510 bp from 3&#x…

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGW-Fray

    Plasmid

    #203979

    Purpose

    GFP-Fray under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Frayed (fray Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAMW-WnkAAxV

    Plasmid

    #203980

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Use

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    RFxV mutated to AAxV

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGB-06-07-Vps34

    Plasmid

    #187989

    Purpose

    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

    Depositor

    Sascha Martens

    Insert

    Vps34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid

    #185061

    Purpose

    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid

    #188722

    Purpose

    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

    Depositor

    Darrell Kotton

    Insert

    TFEB (TFEB Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mutation

    Promoter

    hSP-C

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132622

    Purpose

    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid

    #182342

    Purpose

    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-TagRFP-T-linker-dre-PBD*-FNF
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CNN3A-c007

    Plasmid

    #175457

    Purpose

    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

    Depositor

    Opher Gileadi

    Insert

    CNN3 (CNN3 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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