We narrowed to 23,628 results for: CRISPR

1. qTAG-N-Puro-sTagRFP

   Plasmid

   #207690

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-N-Blast-sTagRFP

   Plasmid

   #207679

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJAT58

   Plasmid

   #204304

   Purpose

   Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE1176

   Plasmid

   #202774

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_tNbEU
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS538

   Plasmid

   #188881

   Purpose

   Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Cas9
   

   Use

   Gateway entry vector

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. rtTA-sg-mGreenLantern332

   Plasmid

   #188483

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   PB_rtTA_BsmBI

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. rtTA-sg-CMV152

   Plasmid

   #188485

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. rtTA-sg-tdTomato166/892

   Plasmid

   #188488

   Purpose

   gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. rtTA-sg-tdTomato90/816

   Plasmid

   #188487

   Purpose

   gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM159

    Plasmid

    #173215

    Purpose

    Expresses dCas9-sfGFP10

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-sfGFP10M2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE Tubb3-2xGFP KI

    Plasmid

    #183443

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 2xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC4 Kcnn2-smFP-myc KI

    Plasmid

    #183438

    Purpose

    FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP myc donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOC2 GFP-Mpp2 KI

    Plasmid

    #183434

    Purpose

    CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC5 smFP-HA-Cacna1e KI

    Plasmid

    #183441

    Purpose

    FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOC2 Gria1-Halo KI

    Plasmid

    #183433

    Purpose

    CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pORANGE Tubb3-3xGFP KI

    Plasmid

    #183444

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 3xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOC2 Tubb3 no donor

    Plasmid

    #183431

    Purpose

    CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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