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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,822 results for: BLI

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Showing: 7041 - 7060 of 17822 results

  1. pcDNA3.1-IARS2-tb

    Plasmid
    #232340
    Purpose
    Expresses human IARS2 with synonymous mutations (to prevent recognition by siRNA) in mammalian cells
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Expression
    Mammalian
    Mutation
    5 synonymous mutations in the siRNA (ACUUGCAGUCAU…
    Promoter
    CMV
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232340-plasmid-map-sequence-id-474527

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  2. pET28a-shortSUMO1

    Plasmid
    #228951
    Purpose
    express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    SUMO1 (SUMO1 Human)
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-17
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228951-plasmid-map-sequence-id-472406

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  4. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

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  5. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

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  6. AICSDP-167:CDH5-mEGFP

    Plasmid
    #217425
    Purpose
    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5
    Depositor
    Allen Institute for Cell Science
    Insert
    CDH5 Homology Arms with mEGFP (CDH5 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217425-plasmid-map-sequence-id-454212

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  14. pSN667

    Plasmid
    #219963
    Purpose
    Expressed sfGFP fused human KIF1Bß(1-721) in insect cells
    Depositor
    Shinsuke Niwa
    Insert
    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    Tags
    sfGFP::2xStrepII tag
    Expression
    Insect
    Mutation
    encodes 1-721
    Promoter
    polyhedrin
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219963-plasmid-map-sequence-id-441237

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  15. pSN668

    Plasmid
    #219962
    Purpose
    Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells
    Depositor
    Shinsuke Niwa
    Insert
    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    Tags
    sfGFP::2xStrepII tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219962-plasmid-map-sequence-id-440846

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  18. pAG426-GAL-APP-CTF

    Plasmid
    #181732
    Purpose
    Galactose inducible expression of APP-CTF
    Depositor
    Alejandro Chavez
    Insert
    APP-CTF (APP Human)
    Expression
    Yeast
    Mutation
    C-terminal fragment (aa 671-770)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    181732-plasmid-map-sequence-id-437192

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  19. PE-SUMO CRYGS Q16C

    Plasmid
    #217674
    Purpose
    For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine
    Promoter
    T7
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217674-plasmid-map-sequence-id-434908

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Showing: 7041 - 7060 of 17822 results