We narrowed to 13,794 results for: CAR

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pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

Plasmid

#107407

Purpose

Lentiviral expression of gRNA resistant PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

sg resistant PBRM1 (PBRM1 Human)

Use

Lentiviral

Mutation

sgRNA resistant

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHC5

Plasmid

#85812

Purpose

To rescue the expression of DHHC5 in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-Puro

Plasmid

#117686

Purpose

The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

Depositor

Luc Leyns

Article

Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

Insert

eSpCas9(1.1)

Use

CRISPR

Tags

PuroR (R166H)

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

U6

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
MBP-TSF-TEVcs-ULK1

Plasmid

#171416

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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keratin 8 (krt8)-H2B-RFP

Plasmid

#105955

Purpose

transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

krt8 (KRT8 Human)

Tags

RFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cre stuffer v4

Plasmid

#158033

Purpose

Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr2 HA

Plasmid

#14692

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox2-3xflag

Plasmid

#206376

Purpose

Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-MAPKAPK2

Plasmid

#82720

Purpose

Expresses human MAPKAPK2

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MAPKAPK2 (MAPKAPK2 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-STAT1

Plasmid

#82722

Purpose

Expresses human STAT1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

STAT1 (STAT1 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 Human, Synthetic, human)

Tags

HiBiT

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Tags

flag

Expression

Mammalian

Mutation

See note below

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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Myc-βarrestin2-LOV-Turbo_pCDNA3

Plasmid

#199671

Purpose

expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Tags

Myc and βarrestin2

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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