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pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

nptII Promoter (Broad)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAG145 HIPK1 3'UTR

Plasmid

#12003

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-9-1

Plasmid

#26317

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-9-1 (MIR9-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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mCerulean3-H2B-6

Plasmid

#55421

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

H2B (H2BC11 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pC1-FGB (FlincG2)

Plasmid

#49204

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG2 (PRKG1 GFP, Bovine)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-205

Plasmid

#26312

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-205 (MIR205 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pNMTMA_MBP-Cav1-His6

Plasmid

#223700

Purpose

To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

MBP-Cav1-His6

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR6

Plasmid

#118997

Purpose

Targeting vector for human AAVS1 with TLR6 reporter insert

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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iCAP-BI155-KanR

Plasmid

#209527

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI155 Cap

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

EGFP

Expression

Insect

Promoter

hsp70

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dUBA

Plasmid

#74205

Purpose

Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed aa 877 to encode for premature stop codon

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pLEX303 GFP-PIK3C2A

Plasmid

#162000

Purpose

Lentiviral expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Human)

Use

Lentiviral

Tags

eGFP

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB808

Plasmid

#47853

Purpose

BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rrnB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO8

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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YFP-DAXX-mSIM1

Plasmid

#134524

Purpose

Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-192

Plasmid

#26311

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-192 (MIR192 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCyterm-mScarlet-I3-NCwt_N1

Plasmid

#189779

Purpose

Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet-I3-NCwt

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-H2B-C-10

Plasmid

#54112

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.2/V5 hsa-mir-125

Plasmid

#26307

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-125 (MIR125A Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only