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pLBM0040

Plasmid

#172819

Purpose

Level 2 module containing HSP21-nYFP deltaPTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0030

Plasmid

#172818

Purpose

Level 2 module containing HSP21-nYFP PTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0020

Plasmid

#172817

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0060

Plasmid

#172821

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBM0070

Plasmid

#172822

Purpose

Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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MBP_Lims (MBP_LIM1234)

Plasmid

#182042

Purpose

For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1234 (PXN Human)

Tags

MBP

Expression

Bacterial

Mutation

LIM domains 1-4 only

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM234

Plasmid

#182039

Purpose

For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM234 (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

LIM domains 2-4 only

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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gMUM2-Citrine

Plasmid

#181965

Purpose

MUM2 protein fused to Citrine, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 (MUM2 Mustard Weed)

Tags

Citrine YFP

Expression

Plant

Promoter

MUM2

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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MUM2SP-Citrine

Plasmid

#181963

Purpose

MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 signal peptide, fused to the Citrine YFP in pAD

Tags

Citrine YFP

Expression

Plant

Promoter

MUM4pro 1.5kb fragment

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

Plasmid

#167921

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo_zhang2.0

Plasmid

#167917

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto_TtoC and hairpin extension

Plasmid

#167920

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EYFP_TtoC and hairpin extension

Plasmid

#167922

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Hygro_TtoC and hairpin extension

Plasmid

#167923

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

Plasmid

#167924

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo_TtoC and hairpin extension

Plasmid

#167925

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro_TtoC and hairpin extension

Plasmid

#167927

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

Plasmid

#167896

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_iRFP713

Plasmid

#167884

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_Puro

Plasmid

#167879

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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