We narrowed to 35,662 results for: puro

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pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

Plasmid

#213118

Purpose

Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

PKAsub-mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

Plasmid

#211687

Purpose

U6-sgRNA-EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

Plasmid

#120564

Purpose

Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

Plasmid

#12153

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

6XHis

Expression

Mammalian

Available Since

July 7, 2006

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag

Plasmid

#236386

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

Plasmid

#214879

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF2-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173230

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

Plasmid

#99277

Purpose

Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mOrange2-IRES-EGFP
Puro

Use

Lentiviral

Tags

EGFP, H2B, IRES, PGK, and mOrange2

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25949

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

Plasmid

#188391

Purpose

Reporter for BCAM expression

Depositor

Markus Frank

Article

Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

Insert

BCAM reporter

Use

Luciferase

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

Plasmid

#211622

Purpose

Rhno1 S141A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S141A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro

Plasmid

#192875

Purpose

Cre-dependent dual reporter

Depositor

Kazuhiro Oka

Insert

Nanoluc-P2A-copGFP

Use

Lentiviral

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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