We narrowed to 31,789 results for: puromycin

1. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AICSDP-63: ACTN2-mEGFP

   Plasmid

   #124607

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1α-Cre-2A-Puro

   Plasmid

   #207743

   Purpose

   Expression of the Cre recombinase linked to a puro selectable marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-2A-Puro
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCW57-MCS1-2A-MCS2

   Plasmid

   #71782

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

   Plasmid

   #31482

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (Sce Target) EF Puro
   

   Use

   Lentiviral

   Mutation

   mCherry has M9S and M16L mutations. This is what …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 Puro shRNA Scramble

   Plasmid

   #162011

   Purpose

   Lentiviral negative control vector containing scramble shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   scramble shRNA
   

   Use

   Lentiviral

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1 EGFP-p62 HRD

   Plasmid

   #207550

   Purpose

   Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-P62
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1 EGFP-LC3B HRD

    Plasmid

    #207551

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-LC3B
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1 EGFP-GABARAPL1 HRD

    Plasmid

    #207552

    Purpose

    Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-GABARAPL1
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEM705 Gsi (Cys)

    Plasmid

    #109373

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM705 Gst (Cys)

    Plasmid

    #109376

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ASAP2f

    Plasmid

    #83960

    Purpose

    Fluorescent reporter for voltage imaging

    Depositor

    Michael Lin

    Article

    Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

    Insert

    ASAP2f
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVXT Sox4

    Plasmid

    #101121

    Purpose

    Sox4 coding region

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SOX4 (Sox4 Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP5

    Plasmid

    #22590

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP5 (USP5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. mito-EcSTH

    Plasmid

    #211921

    Purpose

    Mitochondrial localization of EcSTH

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    mito-EcSTH
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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