We narrowed to 8,836 results for: construct
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Plasmid#202107PurposeLevel 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertGEF promoter
UseSynthetic BiologyMutationPromoter sequence mutated to remove sapI site for…Available SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-47740
Plasmid#202106PurposeLevel 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert47740 promoter
UseSynthetic BiologyMutationPromoter sequence mutated to remove bsaI site for…Available SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-BCA
Plasmid#202105PurposeLevel 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertBCA promoter
UseSynthetic BiologyMutationPromoter sequence mutated to remove sapI sites fo…Available SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-epyC
Plasmid#202104PurposeLevel 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertepyC promoter
UseSynthetic BiologyMutationPromoter sequence mutated to remove bsaI sites fo…Available SinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA
Plasmid#199254PurposeExpression construct encoding the high specificity SpCas9-KARA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KARA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A R1060APromoterCAG promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe NGF-EGFR-FRB-GluGlu neo
Plasmid#192293PurposeRetroviral expression vector for NGF-EGFR-FRB-GluGlu constructDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe NGF-Erbb2-2xFKBP-HA hygro
Plasmid#192291PurposeRetroviral expression vector for NGF-Erbb2-2xFKBP-HA constructDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP76_pU.CAG.Cas9-D10A-K848A.rBGpA
Plasmid#199253PurposeExpression construct encoding the high specificity SpCas9-KA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAPH1Δ536-587
Plasmid#194865PurposeExpress eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.DepositorInsertRAPH1 (RAPH1 Human)
TagseGFPExpressionMammalianMutationRAPH1 with amino acids 536-587 deletedPromoterCMVAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No8
Plasmid#194902PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.8
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No6
Plasmid#194900PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.6
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No7
Plasmid#194901PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.DepositorInsertsgRNA targeting human MECP2 promoter No.7
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB203
Plasmid#169114PurposeTESEC expression vector for mEGFP-Alr fusion constructDepositorInsertmEGFP-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK028
Plasmid#180826PurposeT7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coliDepositorArticleInsertSwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
Tags6X HisExpressionBacterialPromoterT7Available SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK037
Plasmid#180829PurposeT7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coliDepositorArticleAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-scrambled
Plasmid#183455PurposeThis control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα constructDepositorInsertscrambled sgRNA
UseLentiviralPromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry
Plasmid#182381PurposeDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promotersDepositorInsertsTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterHSV TK promoter and SV40 promoterAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1
Plasmid#182380PurposeDual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promotersDepositorInsertsTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterCMV promoter and SV40 promoterAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only