We narrowed to 10,685 results for: plasmids 101

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pET21-pelB-LaG94-1--LaG94-14

Plasmid

#228973

Purpose

For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1--LaG94-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-1

Plasmid

#228974

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-7

Plasmid

#228997

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS1

Plasmid

#170852

Purpose

AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMCherry F8-2

Plasmid

#186034

Purpose

Express VHH F8-2 as a mCherry fusion in mammalian cells

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

mCherry

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Ov-iPGM

Plasmid

#180245

Purpose

Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Onchocerca volvulus iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-2

Plasmid

#228996

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-12

Plasmid

#228999

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJJGL002

Plasmid

#180606

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHEN2 F8-2

Plasmid

#186033

Purpose

Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

6His and Myc

Expression

Bacterial

Promoter

lac

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.RGS4.C-HA

Plasmid

#154847

Purpose

Expresses murine RGS4 protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Regulator of G protein Signaling 4 (Rgs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Fn

Plasmid

#108864

Purpose

FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Type

Empty backbone

Use

CRISPR; Gateway compatible crrna entry clone

Expression

Plant

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.VTN.C-HA

Plasmid

#154849

Purpose

Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Vitronectin (Vtn Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGL421_MLL_promoter

Plasmid

#118695

Purpose

Express MLL/KMT2A promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Promoter

MLL/KMT2A promoter

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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